蔬菜遗传与种质创新利用研究团队针对我国部分蔬菜品种抗性弱、育种资源匮乏、和“洋种子卡脖子”等问题，主要围绕产量、品质、抗性等重要农艺性状，主要开展了对不结球白菜、黄瓜、萝卜、胡萝卜、番茄、草莓的种质资源的收集、鉴定，重要性状关键基因挖掘及功能研究，重要性状形成的分子基础解析，重要农艺性状高效的育种实用的分子标记开发等研究，并将分子育种与常规育种相结合，创制新种质，培育高产、优质、多抗、广适性的蔬菜新品种。2024年度克隆关键基因17个，相关研究成果在Plant Cell、Plant Biotechnology J、Plant Physiol、Horti Resh、Plant Cell Environ等期刊上发表SCI论文43篇，获得授权国家发明专利8项、荷兰发明专利1件，获批植物新品种保护权3件。李英教授获江苏省科技创新协会科技创新奖三等奖。
团队负责人： 侯喜林，陈劲枫
一、研究进展
成功阐明了在胡萝卜肉质根中BCH的酶活性主要依赖于DcBCH1编码酶的作用，DcBCH2起着对DcBCH1酶功能的补充或是部分补偿的作用。成功定位并克隆到四个与黄瓜株型相关基因CsDWF1、CsCLV1、CsABCB19、CsDIM1, 这些基因对于改良黄瓜株型的育种工作具有重要理论指导意义。解析了萝卜基因组进化特征与抽薹开花的转录调控通路，为研究萝卜基因组遗传变异特征构建了重要序列资源平台。解析了CLE信号肽介导的萝卜肉质根维管形成层活性调控的分子通路，揭示了萝卜肉质根形成膨大的分子遗传机制。同时在不结球白菜霜霉病抗性机理研究方面也取得一系列新进展。以上成果为作物精准设计育种提供重要基因资源和有力的理论支撑。
通过在拟南芥中异源过表达BcWRKY1和在白菜中沉默BcWRKY1进一步验证了其是否参与盐胁迫的调控，并阐明了其在盐胁迫的工作模式。创新了染色体工程育种技术体系，建立了单倍体诱导，加快了黄瓜育种进程；构建了森林草莓生长调节因子及其转录共激活因子FveGRF5-FveGIF1、FveGRF10-FveGIF1嵌合基因载体，提高草莓遗传转化效率；构建了突变体fvpils5-1，为提高草莓遗传转化效率提供了新材料；建立了快速、稳定的萝卜毛状根和高效原位遗传转化体系系统，开发了基于VRN1基因的功能标记。在全基因组水平上对胡萝卜 PIF 基因家族进行鉴定并对其进行系统进化、染色体分布、复制事件。
创制了高VC含量的不结球白菜新种质，筛选了薹用苏州青优良自交系新种质1份。创制了系列无刺黄瓜新品系，南水系列黄瓜，其单性结实性好，节成性好，瓜条顺直且产量高，光滑无刺易清洗 ；创制了由异源四倍体‘金瓜’与二倍体黄瓜杂交而来的异源三倍体材料1份，其维生素C含量（185mg/kg）是普通黄瓜的2倍以上，抗病性强、耐高低温和弱光、产量高，适宜于加工类型新品种；创制了由野生酸黄瓜和栽培黄瓜种间杂交和染色体加倍而来的异源四倍体‘金瓜’，除了继承自亲本野生种的抗性基因外，具有耐高温的新表型；利用细胞分裂素受体基因SlHK2的RNAi沉默，创制了耐干旱和高温的番茄新种质2个；创制了番茄细胞分裂素受体基因SlHK4的2个基因编辑耐旱新种质。
培育高产、优质、绿色等满足市场多元化需求的重大新品种，2024年度推广“十三五”以来不结球白菜新品种12.6万亩，品种主要有‘黄玫瑰’、‘夏普雷’。培育优质高产多抗高效的南水系列水果黄瓜，如‘南水6号’；构建新品种优质绿色丰产配套技术体系，加快新品种推广应用。免支架栽培黄瓜新品种‘宁运3号’配套技术获得技术转让；开展优质高抗高效多性状协同改良，培育高产、优质、绿色等满足市场多元化需求的重大新品种。筛选出优质多抗晚抽薹萝卜组合1个、优质抗热萝卜组合1个、优质水果萝卜组合1个，正在进行品种比较试验。
二、科研产出
（一）发表SCI论文
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2. 获奖成果
[1]. 侯喜林，获第十四届“发明创业奖人物奖”，中国发明协会，2024.06.25公示。
[2]. 侯喜林，获江苏省“最美科技工作者”称号，江苏省科学技术协会，2024.05.09公示。
[3]. 李英等，不结球白菜自交不亲和鉴定的SSR分子标记及其应用，获江苏省科技创新协会科技创新奖三等奖，江苏省科技创新协会，2024.11。
[4]. 乔玉山、袁华招、王静、王庆莲、关玲、顾婷婷、陈晓东、蔡伟建、庞夫花、赵密珍、吉沐祥。“草莓优异种质创制与早熟高产品种选育及应用”获2024年江苏省科学技术奖一等奖。
3. 获批专利
[1]. 一种拟南芥种子清选器及清选方法。授权专利号：ZL201811522117.0，完成人：李家曦、侯喜林、范凤翠、刘胜尧、贾宋楠、贾建明、石玉芳，公告日：2024年2月9日。
[2]. 一种十字花科作物花粉电击遗传转化方法。授权专利号：ZL202110886126.3，完成人：张昌伟、杨宇、肖栋、任一鸣、刘同坤、侯喜林、李英、胡春梅、王建军，公告日：2024年6月7日。
[3]. 一种转盘式植物叶片取样器的取样方法。授权专利号：ZL201811522132.5，完成人：李家曦、侯喜林、范凤翠、刘胜尧、刘宏卓、贾宋楠，公告日：2024年10月1日。
[4]. 一种提高森林草莓遗传转化效率的嵌合基因及其载体和应用。授权专利号：ZL202210048726.7，完成人：丁静，田美萍，郭苏平，公告日：2024年2月9日。
[5]. 带有可视化蛋白融合抗生素筛选标记的CRISPR/Cas9载体及其构建方法和应用。授权专利号：ZL202210606954.1，完成人：吴寒，皮颖，李治飞，公告日：2024年3月22日。
[6]. 植物类胡萝卜素合成相关蛋白DcAPRR2及其编码基因与应用。授权专利号：CN 116970053 B，完成人：徐志胜;张凯;熊爱生;王璐，公告日：2024年1月30日。
[7]. 一种混合授粉种间杂交拓宽甜瓜遗传变异的方法。授权专利号：ZL202310057882.4，完成人：钱春桃、郑莉娜、安露西、李季、王瑜晖、程春燕、娄群峰、陈劲枫，公告日：2024年4月26日；
[8]. Method for Inducing Embryo Development by Distant Hybridization for Direct Creation of Double Haploids of Melon。 授权专利号：NL2031462，完成人：钱春桃、李季、张春、娄群峰、陈静、刘麒、黄耀、孙亚亭、陈劲枫、孙博文，公告日：2024年5月14日；
4．获批植物新品种保护权
[1]. 齐心黄 001，品种权号：CNA20191003420，完成人：侯喜林、沈生元、李更雍、李英、张昌伟、钱燕婷、沈海根、王雪萍，授权公告日期：2024年9月18日。
[2]. 绿美风 009，品种权号：CNA20191005276，完成人：李英、侯喜林、刘同坤、肖栋，授权公告日期：2024年9月18日。
南农 L046，品种权号：CNA20191002404，完成人：刘同坤、潘静雯、张蜀宁、张昌伟、侯喜林，授权公告日期：2024年9月18日。
