姓    名：柳李旺

职    称：教授

学    科：蔬菜学

E-mail：nauliulw@yahoo.com.cn

任课名称：园艺植物生物技术、园艺作物育种学、植物组织培养、现代蔬菜科学研究技术（研究生）、Modern Vegetable Science（研究生）      

主要发表论文

Yu Rugang, Wang Yan,Xu Liang, Zhu Xianwen, Zhang Wei, Wang Ronghua, Gong Yiqin,Limera Cecilia,Liu Liwang*. Transcriptome profiling of root microRNAs reveals novel insights into taproot thickening in radish (Raphanussativus L.) .BMC Plant Biology,2015, 15:30,DOI: 10.1186/s12870-015-0427-3(SCI)

Xie Y.^(#), Ye Sh.^(#), Wang Y.^(#), Xu L., Zhu X., Yang J., Feng H., Yu R., Karanja B., Gong Y., Liu L.^(*).Transcriptome-based gene profiling provides novel insights into the characteristics of radish plant response to Cr stress with next-generation sequencing.Frontiers in Plant Science,2015,6:202. doi:10.3389/fpls.2015.00202(SCI)

Sun Xiaochuan, Xu Liang, Wang Yan, Yu Rugang, Zhu Xianwen, LuoXiaobo, Gong Yiqin, Wang Ronghua, Limera Cecilia , Zhang Keyun,Liu Liwang^(*). Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress responseinradish(RaphanussativusL.).BMCGenomics,2015, 16:197(SCI)

Wang Yan^(#), Liu Wei^(#), Shen Hong, Chen Yinglong, Zhai Lulu, Xu Liang, Wang Ronghua, Gong Yiqin, Cecilia Limera, Liu Liwang^*.Identification of radish (Raphanussativus L.) miRNAs and their target genes to explore miRNA-mediated regulatory networks in lead (Pb) stress responses by high-throughput sequencing and degradome analysis.Plant MolBiol Rep ,2015, 33(3):358-376, DOI: 10.1007/s11105-014-0752-y(SCI)

Wang Ronghua, Xu Liang, Zhu Xianwen, Zhai Lulu, Wang Yan, Yu Rugang, Gong Yiqin, Cecilia Limera, LiuLiwang^*. Transcriptome-wide characterization of novel and heat stress-responsive microRNAs in radish (RaphanussativusL.) using next-generation sequencing.Plant MolBiol Rep, 2015,33(4):867-880, DOI 10.1007/s11105-014-0786-1(SCI)

Wang Y.^(#), Shen H.^(#), Xu L.^(#), Liu W., ChenY., GongY., Limera C., Liu L.^(*).Transport, ultrastructural localization and distribution of chemical forms of lead in radish (Raphanussativus L.).Front. Plant Sci. 2015,6:293. doi: 10.3389/fpls.2015.00293(SCI)

NieShanshan, Xu Liang, Wang Yan, HuangDanqiong, MulekeM.Everlyne, Sun Xiaochuan, Wang Ronghua, Xie Yang, Gong Yiqin,Liu Liwang*.Identification of bolting-related microRNAs and their targets reveals complex miRNA-mediated flowering-time regulatory networks in radish (RaphanussativusL.). Sci. Rep. 2015, 5, 14034; doi: 10.1038/srep14034. (SCI)

Xu Liang, WangYan, Liu Wei, Wang Jin, Zhu Xianwen, Zhang Keyun, Yu Rugang, Wang Ronghua, Xie Yang, Zhang Wei, Gong Yiqin, Liu Liwang^*.^. De novo sequencing of root transcriptome reveals complex cadmium-responsive regulatory networks in radish (Raphanussativus L.). Plant Science，2015，236：313-323(SCI)

WuYue^(#), ZhangWei^(#), Xu Liang, Wang Yan, Zhu Xianwen, Li Chao, Liu Liwang^*. Isolation and molecular characterization of nitrite reductase (RsNiR) gene under nitrate treatments in radish.Scientia Horticulturae,2015,193:276-285，DOI: 10.1016/j.scienta.2015.07.016(SCI)

Cecilia Limera, Xu Liang, Zhu Xianwen, FengHaiyang, Liu Liwang*.Characterization of autotetraploid c1 radish (RaphanussativusL.) produced from colchicine induced autotetraploid co plants with their diploid counterparts.International Journal of Current Life Sciences,2015,5(4):563-567

Liu Wei, Xu Liang, Wang Yan, Shen Hong, Zhu Xianwen, Zhang Keyun, Chen Yinglong, Yu Rugang, Limera Cecilia,Liu Liwang^*. Transcriptome-wide analysis of chromium-stress responsive microRNAs to explore miRNA-mediated regulatory networks in radish (RaphanussativusL.).Scientific Reports,2015,5:14024. DOI: 10.1038/srep14024(SCI)

Wang Y., Xu L., Shen H., Wang J., Liu W., Zhu X., Wang R., Sun X., Liu L*.Metabolomic analysis with GC-MS to reveal potential metabolites and biological pathways involved in Pb& Cd stress of radish (RaphanussativusL.). Scientific Reports,2015, 5:18296,DOI: 10.1038/srep18296(SCI)

Cecilia O. Limera^(#), Wang Kang^(#), Xu Liang, Wang Yan, Zhu Xianwen, FengHaiyang, ShaYuhui, Gong Yiqin, Liu Liwang^*.Induction of autotetraploidy using colchicine and its identification in radish (Raphanussativus L.). J HortiSci Biotech, 2016,doi:10. 1080/14620316. 2015.1110993(SCI)

Zhai Lulu, Xu Liang, Wang Yan, Cheng Huan, Chen Yinglong, Gong Yiqin, Liu Liwang^*. Novel and useful genic-SSR markers from de novotranscriptome sequencing of radish (Raphanussativus L.). Molecular Breeding,2014, 33（3）：611-624(SCI)

Wang Xianli^(#), Xu Liang^(#), Song Zhaohui, Zhu Xianwen, Wang Yan, Wang Ronghua, Gong Yiqin, LimeraCecilia,LiuLiwang^*.Resistance gene analogue isolation and RGA-based marker development for identifying downy mildew resistance in radish (RaphanussativusL.).Euphytica,2014,198:289-303.DOI: 10.1007/s10681-014-1105-5 (SCI)

Xu Liang^(#), Jiang Qiuwei^(#), Wu Jian, Wang Yan, Gong Yiqin, Wang Xianli, Limera Cecilia, Liu Liwang^*.Identification and molecular mapping of theRsDmRlocus conferring resistance to downy mildew at seedling stage in radish (Raphanussativus L.). Journal of Integrative Agriculture,2014, 13(11): 2362-2369. Doi:10.1016/S2095-3119(14)60792-9(SCI)

Zhai Lulu, Xu Liang, Wang Yan, Huang Danqiong, Yu Rugang, Cecilia Limera, Gong Yiqin, Liu Liwang^*. Genome-wide identification of embryogenesis-associated microRNAs in Radish (RaphanussativusL.) by high-throughput sequencing.Plant Molecular Biology Reporter,2014,32:900-915.DOI: 10.1007/s11105-014-0700-x(SCI)

Pan Yan, XuYuanyuan, Zhu Xianwen, Liu Zhe, Gong Yiqin, XuLiang,GongMaoyong，LiuLiwang*. Molecular characterization and expression profiles of Myrosinasegene (RsMyr2) in radish (Raphanussativus L.).Journal of Integrative Agriculture,2014, 13(9): 1877-1888.DOI: 10.1016/S2095-3119(13)60644-9(SCI)

Zhang Yanyu^(#)，Xu Liang^(#)，Zhu Xianwen，Yiqin Gong，Xiang Fei，Sun Xiaochuan，Liu Liwang*.Proteomic analysis of heat stress response in leaves of radish (Raphanussativus L.).Plant Molecular Biology Reporter,2013,31(1):195-203,DOI:10.1007/s11105-012-0486-7(SCI)

Xu Liang,Wang Yan,XuYuanyuan,WangLiangjuWang,Zhai Lulu, Zhu Xianwen, Gong Yiqin, Ye Shan,LiuLiwang^*.Identification and characterization of novel and conserved microRNAs in radish (RaphanussativusL.) using high-throughput sequencing.Plant Science, 2013,201-202: 108-114(SCI)

Ye Shan，Wang Yan, Huang Danqiong, Li Jianbin, Gong Yiqin, Xu Liang, Liu Liwang*. Genetic purity testing of F₁ hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata).ScientiaHorticulturae,2013,155: 92-96(SCI)

Wang Y., Xu L., Chen Y., Shen H., Gong Y., Limera C., Liu L.*. Transcriptome profiling of radish (Raphanussativus L.) root and identification of genes involved in response to lead (Pb) stress with next generation sequencing. PLoS ONE,2013,8(6):e66539. doi:10.1371/journal.pone.0066539(SCI)

Xu Yao, Zhu Xianwen,Chen Yinglong,Gong Yiqin,Liu Liwang*, Expression profiling of genes involved in ascorbate biosynthesis and recycling during fleshy root development in radish. Plant Physiology and Biochemistry, 2013,70：269-277(SCI)

Wang Yan^(#),Pan Yan^(#),Liu Zhe,Zhu Xianwen,Zhai Lulu,XuLiang,YuRugang,Gong Yiqin, Liu Liwang^*. De novo transcriptome sequencing of radish (Raphanussativus L.) and analysis of major genes involved in glucosinolate metabolism. BMC genomics, 2013, 14:836，DOI:10.1186/1471-2164-14-836(SCI)

Xu L., Wang Y., Zhai L., Xu Y., Wang L., ZhuX., Gong Y., Yu R., LimeraC., Liu L.^*. Genome-wide identification and characterization of cadmium-responsive microRNAs and their targets in radish (Raphanussativus L.) roots.J Exp Bot,2013,64(14):4271-4287(SCI)

Cheng D.^(#), Zhang F.^(#), Liu L.*, Xu L., Chen Y., Wang X., Limera C, Yu R., Gong Y.*.TRAP markers generated with resistant gene analog sequences and their application to genetic diversity analysis of radish germplasm.ScientiaHorticulturae, 2013，161:153-159 (SCI)

Xinju Sun，Yan Wang，Yinglong Chen，Liang Xu，Lina Jiang，Yiqin Gong，Liangju Wang，Liwang Liu^* Differential gene expression profiling of Ogura CMS line and its maintainer in radish (Raphanussativus L.). ActaPhysiologiaePlantarum,2013, 35(12):3413-3425 (SCI)

Xu Liang, Wang Liangju, Gong Yiqin, Dai Wenhao, Wang Yan, Zhu Xianwen, Wen Tiancai, Liu Liwang^*. Genetic linkage map construction and QTL mapping of cadmium accumulation in radish. TheorAppl Genet,2012,125(4):659-670,DOI 10.1007/s00122-012-1858-y (SCI)

Sun Xinju, Liu Yang, Wang Liangju, Xianwen Zhu, Gong Yiqin, Liu Liwang^*.Molecular characterization of the Rs-Rf₁ gene and molecular marker-assisted development of elite radish (Raphanussativus L.) CMS lines with a functional marker for fertility restoration. Mol Breeding,2012,30:1727-1736.DOI 10.1007/s11032-012-9756-1(SCI)

Jiang Lina, Wang Liangju, Liu Liwang*, Zhu Xianwen, Zhai Lulu, Gong Yiqin*. Development and characterization of cDNA library based novel EST-SSR marker in radish (Raphanussativus L.).ScientiaHorticulturae, 2012,140 :164-172 (SCI)

XuYuanyuan, Zhu Xianwen, Gong Yiqin*, XuLiang, Wang Yan, Liu Liwang*.Evaluation of reference genes for gene expression studies in radish (RaphanussativusL.) using quantitative real-time PCR.Biochemical and Biophysical Research Communication ,2012,424：398-403，DOI 10.1016/j.bbrc.2012.06.119 (SCI)

Wang K, ShaY, Gong Y, Xu L,Liu L*,Zhu X, Huang D. Karyotype analysis and molecular markers identification of radish (Raphanussativus L.) germplasm.ActaHorticulturae(ISHS),2012,929:214-221 (ISTP)

ChuR., WangF.,Li Sh.,Gong Y.,Zhu X.,Xu L.,PuX.,LiuL.*.Application of molecular makers in genetic diversity analysis of radish germplasm with different characters of Cd accumulation.Bioinformatics and Biomedical Engineering, 2010,1-4.Digital Object Identifier：10.1109/ICBBE.2010.5517385, (EI)

著作教材

柳李旺(副主编).《萝卜优质高效四季栽培》，中国科学技术文献出版社,2001.8

柳李旺(副主编).《中国萝卜》(国家科学技术学术著作出版基金资助出版).中国科学技术文献出版社,2005.7

柳李旺(参编).《园艺学各论》面向二十一世纪教材，中国农业出版社，2004

陈火英，柳李旺(主编). 《种子种苗学》，上海交通大学出版社，2011.7

柳李旺(参编).《蔬菜育种与种子生产》，南京大学出版社，2000.5

柳李旺(副主编).《种子生产学实验技术》，科学出版社,2014.7

发明专利：

萝卜霜霉病抗性基因紧密连锁的分子标记, ZL. 201210528077.7 1/6

一种利用标记辅助选择进行萝卜CMS雄性不育系培育的方法ZL.201010266351.9 1/6

一种获得萝卜单倍体的育种方法 ZL. 200810123895.2 3/5

一种基于PCR技术的番茄杂交种纯度检测方法 ZL.200710020044.0 1/4

一种甘蓝种子遗传纯度的快速鉴定方法 ZL.200710020045.5 1/4

一种糯玉米杂交种遗传纯度的快速鉴定方法 ZL. 201110351885.6 1/6

一种基于PCR的甜瓜杂交种种子遗传纯度快速鉴定方法 ZL 201210192805.1 1/6

品种鉴定：

‘南春白5号’萝卜，通过江苏省农作物新品种鉴定（证书编号2010-2-3），江苏省农业委员会公告(第四十八次农作物品种审定会议鉴定公告）(第25号)，2010.11.8

‘南春白6号’萝卜，通过江苏省农作物新品种鉴定（证书编号2010-2-4），江苏省农业委员会公告(第四十八次农作物品种审定会议鉴定公告）(第25号)，2010.11.8

‘南热红3号’萝卜，通过江苏省农作物新品种鉴定（证书编号2014-1-13）[江苏省农业委员会公告(第55次农作物品种审定会议鉴定公告）(2014年第12号)]，2014.6.20

‘南热白4号’萝卜，通过江苏省农作物新品种鉴定（证书编号2014-1-18）[江苏省农业委员会第55次农作物品种审定会议鉴定公告）(2014年第12号)]，2014.6.20

标准制定：

江苏省地方标准：

《蔬菜作物种子遗传纯度DNA分子检测技术规程》（DB32/T 2390-2013, 发布日期: 2013-12-30,国家标准化管理委员备案号 41075-2014）

《‘南春白5号’萝卜品种》(DB32/T 2758-2015, 发布日期:2015-02-15，国家标准化管理委员备案号 44838-2015)

《‘南春白6号’萝卜生产技术规程》(DB32/T 2759-2015, 发布日期:2015-02-15,国家标准化管理委员备案号44839-2015)

《萝卜品种试验技术规程》(DB32/T 2760-2015, 发布日期:2015-02-15，国家标准化管理委员备案号44840-2015)

科研项目：

国家自然科学基金萝卜镉吸收累积性状关键基因鉴定与功能分析 (31372064 ), 2014.1-2017.12

国家自然科学基金萝卜抽薹开花关键基因FLC/FT遗传特性与功能分析（31171956）(Co-PI)，2012.1-2015.12

国家自然科学基金萝卜Cd吸收累积性状的遗传特性与相关基因表达分析（30972006），2009.9-2010.12

国家自然科学基金萝卜晚抽薹性状遗传标记与相关基因的分离克隆（30300238），2004.1-2006.12

国家自然科学基金萝卜肉质直根形成膨大的遗传学机理研究（30571193），2006.1-2008.12

国家科技支撑计划课题萝卜雄性不育育种技术研究与新品种选育（2008BADB1B03），2008.1-2010.12

国家高技术研究发展计划（863计划）南方多湿地区蔬菜优质多抗分子聚合育种（萝卜专题）(2008AA10Z150), 2008-2010

国家科技部农业科技成果转化资金项目优质多抗春萝卜新品种示范与推广（2011GB23600006），2011.4-2013.11

国家科技支撑计划子课题设施专用耐抽薹春萝卜高效育种体系建立及新品种选育2009BADB8B03-3,2009.1 -2011.12

国家科技支撑计划子课题十字花科蔬菜杂种优势利用与新品种选育（2012BAD02B01），2012.1- 2016.12

蔬菜学（NCET-10-0473）教育部新世纪优秀人才支持计划资助, 2010.1-2012.12

中央高校基本科研业务费自主创新重点研究项目萝卜Cd吸收积累性状QTLs定位与相关功能基因鉴定(KYZ201209). 2012.6-2014.12

江苏省农业科技自主创新资金项目, 优质、多抗、耐热萝卜新品种选育[CX(13)2007],2013.7-2016.7

江苏省科技支撑计划项目优质多抗专用萝卜新品种选育（BE2010328），2010.10 -2014.6

江苏省高技术研究计划优质抗病设施栽培萝卜新品种选育与育种技术研究BG2005314， 2005-2009

江苏省科技支撑计划项目江苏主要蔬菜作物品种种子遗传纯度分子检测研究（BE2008312），2008-2011

江苏省农业种质资源基因库项目江苏省十字花科蔬菜种质资源基因库建设（萝卜专题）[No. sx(2007)g13] 2007.7-2009.12

江苏省自然科学基金(创新人才) 萝卜晚抽薹性状分子遗传学机理研究BK2004418 2005.1-2007.12

教育部科学技术研究重点项目萝卜镉吸收累积特性的遗传学特征鉴定(No.108068) ，2008.1-2010.12

上海农业“四新”重点项目（招标）出口西兰花、生菜安全生产技术开发[农字（2001-3-1）] ,2002-2005

上海市科技兴农重点攻关项目主要蔬菜作物种子种苗质量的高效快速检测技术研究沪农科攻字（2004）第9-1号2004. 11-2009.11

科技奖励：

萝卜种质鉴定评价与优良品种创新利用，江苏省科学技术二等奖，江苏省人民政府，2011.2（第1完成人）

西兰花分子育种、高效栽培与加工技术研究及推广，南通市科技进步一等奖，南通市人民政府，2010.12（第4完成人）

西兰花产业化关键技术的开发与推广，江苏省农业技术推广奖三等奖，江苏省人民政府，2011.4.23 （第10完成人）

江苏省青年科技奖 中共江苏省委组织部,江苏省人力资源和社会保障厅,江苏省科学技术协会，2012.12

江苏省第四期“333高层次人才培养工程”第三层次培养对象（中青年科学技术带头人），江苏省人才工作领导小组,2011.9.

江苏省优秀博士学位论文指导教师，2014